Construct: shRNA TRCN0000436767

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014407.3-880s21c1
DNA Barcode:
ACCCGTGTCTTCAGGTGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNMB3 (27094)
Gene Description:
potassium calcium-activated channel subfamily M regulatory beta subunit 3
Transcript:
RefSeq NM_014407.3 (CURRENT)
Match location:
Position 880 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436767)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCGTGTCTTCAGGTGTTTG
Hairpin Sequence:
5'-CCGG-ACCCGTGTCTTCAGGTGTTTG-CTCGAG-CAAACACCTGAAGACACGGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCGTGTCTTCAGGTGTTTGCTCGAGCAAACACCTGAAGACACGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCGTGTCTTCAGGTGTTTGCTCGAGCAAACACCTGAAGACACGGGT-3'

Other clones with same target sequence:

(none)