Construct: shRNA TRCN0000436817
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_020637.1-262s21c1
- DNA Barcode:
- GCAGTGTCCTCAGGCTTCTAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGF22 (27006)
- Gene Description:
- fibroblast growth factor 22
- Transcript:
- RefSeq NM_020637.1 (CURRENT)
- Match location:
- Position 262 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000436817)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGTGTCCTCAGGCTTCTAC
- Hairpin Sequence:
-
5'-CCGG-GCAGTGTCCTCAGGCTTCTAC-CTCGAG-GTAGAAGCCTGAGGACACTGC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGTGTCCTCAGGCTTCTACCTCGAGGTAGAAGCCTGAGGACACTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGTGTCCTCAGGCTTCTACCTCGAGGTAGAAGCCTGAGGACACTGC-3'
Other clones with same target sequence:
(none)
