Construct: shRNA TRCN0000436825

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017882.2-637s21c1
DNA Barcode:
AGACGCTGATCGACTCCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLN6 (54982)
Gene Description:
CLN6 transmembrane ER protein
Transcript:
RefSeq NM_017882.2 (NON-CURRENT)
Match location:
Position 637 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436825)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACGCTGATCGACTCCTTTG
Hairpin Sequence:
5'-CCGG-AGACGCTGATCGACTCCTTTG-CTCGAG-CAAAGGAGTCGATCAGCGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACGCTGATCGACTCCTTTGCTCGAGCAAAGGAGTCGATCAGCGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACGCTGATCGACTCCTTTGCTCGAGCAAAGGAGTCGATCAGCGTCT-3'

Other clones with same target sequence:

(none)