Construct: shRNA TRCN0000436861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018384.3-1283s21c1
DNA Barcode:
GGACCCTGGAGCACTTCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GIMAP5 (55340)
Gene Description:
GTPase, IMAP family member 5
Transcript:
RefSeq NM_018384.3 (NON-CURRENT)
Match location:
Position 1283 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACCCTGGAGCACTTCTAAT
Hairpin Sequence:
5'-CCGG-GGACCCTGGAGCACTTCTAAT-CTCGAG-ATTAGAAGTGCTCCAGGGTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACCCTGGAGCACTTCTAATCTCGAGATTAGAAGTGCTCCAGGGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACCCTGGAGCACTTCTAATCTCGAGATTAGAAGTGCTCCAGGGTCC-3'

Other clones with same target sequence:

(none)