Construct: shRNA TRCN0000436886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008609.3-638s21c1
DNA Barcode:
GGCCATGGTACAGCGTCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mmp15 (17388)
Gene Description:
matrix metallopeptidase 15
Transcript:
RefSeq NM_008609.3 (NON-CURRENT)
Match location:
Position 638 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCATGGTACAGCGTCTAAA
Hairpin Sequence:
5'-CCGG-GGCCATGGTACAGCGTCTAAA-CTCGAG-TTTAGACGCTGTACCATGGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCATGGTACAGCGTCTAAACTCGAGTTTAGACGCTGTACCATGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCATGGTACAGCGTCTAAACTCGAGTTTAGACGCTGTACCATGGCC-3'

Other clones with same target sequence:

(none)