Construct: shRNA TRCN0000436962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133721.2-2778s21c1
DNA Barcode:
GGGTAACTGCTCTCTACAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itga9 (104099)
Gene Description:
integrin alpha 9
Transcript:
RefSeq NM_133721.2 (CURRENT)
Match location:
Position 2778 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTAACTGCTCTCTACAAAG
Hairpin Sequence:
5'-CCGG-GGGTAACTGCTCTCTACAAAG-CTCGAG-CTTTGTAGAGAGCAGTTACCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTAACTGCTCTCTACAAAGCTCGAGCTTTGTAGAGAGCAGTTACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTAACTGCTCTCTACAAAGCTCGAGCTTTGTAGAGAGCAGTTACCC-3'

Other clones with same target sequence:

(none)