Construct: shRNA TRCN0000436975

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053166.2-922s21c1
DNA Barcode:
GGGCAATGCAACTCAACAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim7 (94089)
Gene Description:
tripartite motif-containing 7
Transcript:
RefSeq NM_053166.2 (CURRENT)
Match location:
Position 922 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436975)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCAATGCAACTCAACAATG
Hairpin Sequence:
5'-CCGG-GGGCAATGCAACTCAACAATG-CTCGAG-CATTGTTGAGTTGCATTGCCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCAATGCAACTCAACAATGCTCGAGCATTGTTGAGTTGCATTGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCAATGCAACTCAACAATGCTCGAGCATTGTTGAGTTGCATTGCCC-3'

Other clones with same target sequence:

(none)