Construct: shRNA TRCN0000437009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144845.3-1719s21c1
DNA Barcode:
TCTTCAGAGGCCTTCCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugt3a2 (223337)
Gene Description:
UDP glycosyltransferases 3 family, polypeptide A2
Transcript:
RefSeq NM_144845.3 (CURRENT)
Match location:
Position 1719 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTCAGAGGCCTTCCGTAAT
Hairpin Sequence:
5'-CCGG-TCTTCAGAGGCCTTCCGTAAT-CTCGAG-ATTACGGAAGGCCTCTGAAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTCAGAGGCCTTCCGTAATCTCGAGATTACGGAAGGCCTCTGAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTCAGAGGCCTTCCGTAATCTCGAGATTACGGAAGGCCTCTGAAGA-3'

Other clones with same target sequence:

(none)