Construct: shRNA TRCN0000437015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007813.2-1303s21c1
DNA Barcode:
TCTACCCTTCTCCACAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2b13 (13089)
Gene Description:
cytochrome P450, family 2, subfamily b, polypeptide 13
Transcript:
RefSeq NM_007813.2 (CURRENT)
Match location:
Position 1303 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTACCCTTCTCCACAGGAAA
Hairpin Sequence:
5'-CCGG-TCTACCCTTCTCCACAGGAAA-CTCGAG-TTTCCTGTGGAGAAGGGTAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTACCCTTCTCCACAGGAAACTCGAGTTTCCTGTGGAGAAGGGTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTACCCTTCTCCACAGGAAACTCGAGTTTCCTGTGGAGAAGGGTAGA-3'

Other clones with same target sequence:

(none)