Construct: shRNA TRCN0000437022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022898.1-1328s21c1
DNA Barcode:
AGAGCAAGTCGTGCGAGTTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCL11B (64919)
Gene Description:
BAF chromatin remodeling complex subunit BCL11B
Transcript:
RefSeq NM_022898.1 (NON-CURRENT)
Match location:
Position 1328 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCAAGTCGTGCGAGTTCT
Hairpin Sequence:
5'-CCGC-AGAGCAAGTCGTGCGAGTTCT-CTCGAG-AGAACTCGCACGACTTGCTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCAAGTCGTGCGAGTTCTCTCGAGAGAACTCGCACGACTTGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCAAGTCGTGCGAGTTCTCTCGAGAGAACTCGCACGACTTGCTCT-3'

Other clones with same target sequence:

(none)