Construct: shRNA TRCN0000437061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177708.5-1901s21c1
DNA Barcode:
GGCACGCAAAGTGAGTCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rtn4rl1 (237847)
Gene Description:
reticulon 4 receptor-like 1
Transcript:
RefSeq NM_177708.5 (CURRENT)
Match location:
Position 1901 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCACGCAAAGTGAGTCATTA
Hairpin Sequence:
5'-CCGG-GGCACGCAAAGTGAGTCATTA-CTCGAG-TAATGACTCACTTTGCGTGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCACGCAAAGTGAGTCATTACTCGAGTAATGACTCACTTTGCGTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCACGCAAAGTGAGTCATTACTCGAGTAATGACTCACTTTGCGTGCC-3'

Other clones with same target sequence:

(none)