Construct: shRNA TRCN0000437094
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010945.2-1287s21c1
- DNA Barcode:
- TGCAACCTTCCGAGATCTTAG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Nsmaf (18201)
- Gene Description:
- neutral sphingomyelinase (N-SMase) activation associated factor
- Transcript:
- RefSeq NM_010945.2 (CURRENT)
- Match location:
- Position 1287 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000437094)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGCAACCTTCCGAGATCTTAG
- Hairpin Sequence:
-
5'-CCGG-TGCAACCTTCCGAGATCTTAG-CTCGAG-CTAAGATCTCGGAAGGTTGCA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGCAACCTTCCGAGATCTTAGCTCGAGCTAAGATCTCGGAAGGTTGCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGCAACCTTCCGAGATCTTAGCTCGAGCTAAGATCTCGGAAGGTTGCA-3'
Other clones with same target sequence:
(none)
