Construct: shRNA TRCN0000437131

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025078.4-279s21c1
DNA Barcode:
AGTATCGGGACATTCGCAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC66A2 (80148)
Gene Description:
solute carrier family 66 member 2
Transcript:
RefSeq NM_025078.4 (NON-CURRENT)
Match location:
Position 279 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437131)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTATCGGGACATTCGCAGGA
Hairpin Sequence:
5'-CCGG-AGTATCGGGACATTCGCAGGA-CTCGAG-TCCTGCGAATGTCCCGATACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTATCGGGACATTCGCAGGACTCGAGTCCTGCGAATGTCCCGATACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTATCGGGACATTCGCAGGACTCGAGTCCTGCGAATGTCCCGATACT-3'

Other clones with same target sequence:

(none)