Construct: shRNA TRCN0000437256

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032342.1-760s21c1
DNA Barcode:
GGAGCGTAGTGTGAGCCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PGAP4 (84302)
Gene Description:
post-GPI attachment to proteins GalNAc transferase 4
Transcript:
RefSeq NM_032342.1 (NON-CURRENT)
Match location:
Position 760 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437256)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGCGTAGTGTGAGCCATTT
Hairpin Sequence:
5'-CCGG-GGAGCGTAGTGTGAGCCATTT-CTCGAG-AAATGGCTCACACTACGCTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGCGTAGTGTGAGCCATTTCTCGAGAAATGGCTCACACTACGCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGCGTAGTGTGAGCCATTTCTCGAGAAATGGCTCACACTACGCTCC-3'

Other clones with same target sequence:

(none)