Construct: shRNA TRCN0000437277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024884.2-519s21c1
DNA Barcode:
CCAGACTTCAGGCCCTATATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
L2HGDH (79944)
Gene Description:
L-2-hydroxyglutarate dehydrogenase
Transcript:
RefSeq NM_024884.2 (NON-CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACTTCAGGCCCTATATG
Hairpin Sequence:
5'-CCGG-CCAGACTTCAGGCCCTATATG-CTCGAG-CATATAGGGCCTGAAGTCTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACTTCAGGCCCTATATGCTCGAGCATATAGGGCCTGAAGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACTTCAGGCCCTATATGCTCGAGCATATAGGGCCTGAAGTCTGG-3'

Other clones with same target sequence:

(none)