Construct: shRNA TRCN0000437317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026458.4-4496s21c1
DNA Barcode:
CATGACCTCTCGGGAGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abca14 (67928)
Gene Description:
ATP-binding cassette, sub-family A (ABC1), member 14
Transcript:
RefSeq NM_026458.4 (CURRENT)
Match location:
Position 4496 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGACCTCTCGGGAGATATT
Hairpin Sequence:
5'-CCGG-CATGACCTCTCGGGAGATATT-CTCGAG-AATATCTCCCGAGAGGTCATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGACCTCTCGGGAGATATTCTCGAGAATATCTCCCGAGAGGTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGACCTCTCGGGAGATATTCTCGAGAATATCTCCCGAGAGGTCATG-3'

Other clones with same target sequence:

(none)