Construct: shRNA TRCN0000437412

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032230.2-1403s21c1
DNA Barcode:
GATGGTGCTGTGGTCGTAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
METTL25 (84190)
Gene Description:
methyltransferase like 25
Transcript:
RefSeq NM_032230.2 (NON-CURRENT)
Match location:
Position 1403 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437412)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGTGCTGTGGTCGTAATG
Hairpin Sequence:
5'-CCGG-GATGGTGCTGTGGTCGTAATG-CTCGAG-CATTACGACCACAGCACCATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGTGCTGTGGTCGTAATGCTCGAGCATTACGACCACAGCACCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGTGCTGTGGTCGTAATGCTCGAGCATTACGACCACAGCACCATC-3'

Other clones with same target sequence:

(none)