Construct: shRNA TRCN0000437427

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029466.4-366s21c1
DNA Barcode:
ATCGGTGGTCAAGAATCTCTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arl5b (75869)
Gene Description:
ADP-ribosylation factor-like 5B
Transcript:
RefSeq NM_029466.4 (CURRENT)
Match location:
Position 366 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437427)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGGTGGTCAAGAATCTCTG
Hairpin Sequence:
5'-CCGG-ATCGGTGGTCAAGAATCTCTG-CTCGAG-CAGAGATTCTTGACCACCGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGGTGGTCAAGAATCTCTGCTCGAGCAGAGATTCTTGACCACCGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGGTGGTCAAGAATCTCTGCTCGAGCAGAGATTCTTGACCACCGAT-3'

Other clones with same target sequence:

(none)