Construct: shRNA TRCN0000437430

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030758.3-3286s21c1
DNA Barcode:
CGCACTTAGCAGACAGCTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OSBP2 (23762)
Gene Description:
oxysterol binding protein 2
Transcript:
RefSeq NM_030758.3 (NON-CURRENT)
Match location:
Position 3286 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437430)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCACTTAGCAGACAGCTTTC
Hairpin Sequence:
5'-CCGG-CGCACTTAGCAGACAGCTTTC-CTCGAG-GAAAGCTGTCTGCTAAGTGCG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCACTTAGCAGACAGCTTTCCTCGAGGAAAGCTGTCTGCTAAGTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCACTTAGCAGACAGCTTTCCTCGAGGAAAGCTGTCTGCTAAGTGCG-3'

Other clones with same target sequence:

(none)