Construct: shRNA TRCN0000437483

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181712.4-434s21c1
DNA Barcode:
TCCGAAGAGCCACCCTTATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KANK4 (163782)
Gene Description:
KN motif and ankyrin repeat domains 4
Transcript:
RefSeq NM_181712.4 (NON-CURRENT)
Match location:
Position 434 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437483)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGAAGAGCCACCCTTATTC
Hairpin Sequence:
5'-CCGG-TCCGAAGAGCCACCCTTATTC-CTCGAG-GAATAAGGGTGGCTCTTCGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGAAGAGCCACCCTTATTCCTCGAGGAATAAGGGTGGCTCTTCGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGAAGAGCCACCCTTATTCCTCGAGGAATAAGGGTGGCTCTTCGGA-3'

Other clones with same target sequence:

(none)