Construct: shRNA TRCN0000437524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177613.2-1112s21c1
DNA Barcode:
CCACTCAGCTTTCAGATGATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdc34 (216150)
Gene Description:
cell division cycle 34
Transcript:
RefSeq NM_177613.2 (CURRENT)
Match location:
Position 1112 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCAGCTTTCAGATGATG
Hairpin Sequence:
5'-CCGG-CCACTCAGCTTTCAGATGATG-CTCGAG-CATCATCTGAAAGCTGAGTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCAGCTTTCAGATGATGCTCGAGCATCATCTGAAAGCTGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCAGCTTTCAGATGATGCTCGAGCATCATCTGAAAGCTGAGTGG-3'

Other clones with same target sequence:

(none)