Construct: shRNA TRCN0000437535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018918.2-695s21c1
DNA Barcode:
TCCTCGACGCAAACGACAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHGA5 (56110)
Gene Description:
protocadherin gamma subfamily A, 5
Transcript:
RefSeq NM_018918.2 (CURRENT)
Match location:
Position 695 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTCGACGCAAACGACAATG
Hairpin Sequence:
5'-CCGG-TCCTCGACGCAAACGACAATG-CTCGAG-CATTGTCGTTTGCGTCGAGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTCGACGCAAACGACAATGCTCGAGCATTGTCGTTTGCGTCGAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTCGACGCAAACGACAATGCTCGAGCATTGTCGTTTGCGTCGAGGA-3'

Other clones with same target sequence:

(none)