Construct: shRNA TRCN0000437563
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008292.3-491s21c1
- DNA Barcode:
- CAACTTTGGCCAGGCGAATTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Hsd17b4 (15488)
- Gene Description:
- hydroxysteroid (17-beta) dehydrogenase 4
- Transcript:
- RefSeq NM_008292.3 (NON-CURRENT)
- Match location:
- Position 491 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000437563)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAACTTTGGCCAGGCGAATTA
- Hairpin Sequence:
-
5'-CCGG-CAACTTTGGCCAGGCGAATTA-CTCGAG-TAATTCGCCTGGCCAAAGTTG-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAACTTTGGCCAGGCGAATTACTCGAGTAATTCGCCTGGCCAAAGTTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAACTTTGGCCAGGCGAATTACTCGAGTAATTCGCCTGGCCAAAGTTG-3'
Other clones with same target sequence:
(none)
