Construct: shRNA TRCN0000437768
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_021012.4-2148s21c1
- DNA Barcode:
- TGCAACGGCCTCAGAAGTTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KCNJ12 (3768)
- Gene Description:
- potassium inwardly rectifying channel subfamily J member 12
- Transcript:
- RefSeq NM_021012.4 (NON-CURRENT)
- Match location:
- Position 2148 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000437768)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGCAACGGCCTCAGAAGTTTG
- Hairpin Sequence:
-
5'-CCGG-TGCAACGGCCTCAGAAGTTTG-CTCGAG-CAAACTTCTGAGGCCGTTGCA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGCAACGGCCTCAGAAGTTTGCTCGAGCAAACTTCTGAGGCCGTTGCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGCAACGGCCTCAGAAGTTTGCTCGAGCAAACTTCTGAGGCCGTTGCA-3'
Other clones with same target sequence:
(none)
