Construct: shRNA TRCN0000438009
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_173689.5-1316s21c1
- DNA Barcode:
- TGCCACCTGCATCCCTATCTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CRB2 (286204)
- Gene Description:
- crumbs cell polarity complex component 2
- Transcript:
- RefSeq NM_173689.5 (NON-CURRENT)
- Match location:
- Position 1316 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000438009)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGCCACCTGCATCCCTATCTT
- Hairpin Sequence:
-
5'-CCGG-TGCCACCTGCATCCCTATCTT-CTCGAG-AAGATAGGGATGCAGGTGGCA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGCCACCTGCATCCCTATCTTCTCGAGAAGATAGGGATGCAGGTGGCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGCCACCTGCATCCCTATCTTCTCGAGAAGATAGGGATGCAGGTGGCA-3'
Other clones with same target sequence:
(none)
