Construct: shRNA TRCN0000438029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007317.1-879s21c1
DNA Barcode:
CAACAAGGGCCTTCGGCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIF22 (3835)
Gene Description:
kinesin family member 22
Transcript:
RefSeq NM_007317.1 (NON-CURRENT)
Match location:
Position 879 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAAGGGCCTTCGGCTAAA
Hairpin Sequence:
5'-CCGG-CAACAAGGGCCTTCGGCTAAA-CTCGAG-TTTAGCCGAAGGCCCTTGTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAAGGGCCTTCGGCTAAACTCGAGTTTAGCCGAAGGCCCTTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAAGGGCCTTCGGCTAAACTCGAGTTTAGCCGAAGGCCCTTGTTG-3'

Other clones with same target sequence:

(none)