Construct: shRNA TRCN0000438140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007155.5-1128s21c1
DNA Barcode:
GCTCGGCTGACATCTGTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZP3 (7784)
Gene Description:
zona pellucida glycoprotein 3
Transcript:
RefSeq NM_007155.5 (NON-CURRENT)
Match location:
Position 1128 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGGCTGACATCTGTCAAT
Hairpin Sequence:
5'-CCGG-GCTCGGCTGACATCTGTCAAT-CTCGAG-ATTGACAGATGTCAGCCGAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGGCTGACATCTGTCAATCTCGAGATTGACAGATGTCAGCCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGGCTGACATCTGTCAATCTCGAGATTGACAGATGTCAGCCGAGC-3'

Other clones with same target sequence:

(none)