Construct: shRNA TRCN0000438177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138422.1-1368s21c1
DNA Barcode:
ACAAGCCTGACCGTGGATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAT3 (113179)
Gene Description:
adenosine deaminase tRNA specific 3
Transcript:
RefSeq NM_138422.1 (NON-CURRENT)
Match location:
Position 1368 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAGCCTGACCGTGGATTTC
Hairpin Sequence:
5'-CCGG-ACAAGCCTGACCGTGGATTTC-CTCGAG-GAAATCCACGGTCAGGCTTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAGCCTGACCGTGGATTTCCTCGAGGAAATCCACGGTCAGGCTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAGCCTGACCGTGGATTTCCTCGAGGAAATCCACGGTCAGGCTTGT-3'

Other clones with same target sequence:

(none)