Construct: shRNA TRCN0000438216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183357.1-2264s21c1
DNA Barcode:
AGCAGGTAGACGACCGATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADCY5 (111)
Gene Description:
adenylate cyclase 5
Transcript:
RefSeq NM_183357.1 (NON-CURRENT)
Match location:
Position 2264 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGGTAGACGACCGATTTG
Hairpin Sequence:
5'-CCGG-AGCAGGTAGACGACCGATTTG-CTCGAG-CAAATCGGTCGTCTACCTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGGTAGACGACCGATTTGCTCGAGCAAATCGGTCGTCTACCTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGGTAGACGACCGATTTGCTCGAGCAAATCGGTCGTCTACCTGCT-3'

Other clones with same target sequence:

(none)