Construct: shRNA TRCN0000438272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009616.3-2032s21c1
DNA Barcode:
GTGTGGCCACAACCATATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adam19 (11492)
Gene Description:
a disintegrin and metallopeptidase domain 19 (meltrin beta)
Transcript:
RefSeq NM_009616.3 (NON-CURRENT)
Match location:
Position 2032 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTGGCCACAACCATATTTG
Hairpin Sequence:
5'-CCGG-GTGTGGCCACAACCATATTTG-CTCGAG-CAAATATGGTTGTGGCCACAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTGGCCACAACCATATTTGCTCGAGCAAATATGGTTGTGGCCACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTGGCCACAACCATATTTGCTCGAGCAAATATGGTTGTGGCCACAC-3'

Other clones with same target sequence:

(none)