Construct: shRNA TRCN0000438284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000335.4-5894s21c1
DNA Barcode:
AGAGGTGTCGGCCATGGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCN5A (6331)
Gene Description:
sodium voltage-gated channel alpha subunit 5
Transcript:
RefSeq NM_000335.4 (CURRENT)
Match location:
Position 5894 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGTGTCGGCCATGGTTAT
Hairpin Sequence:
5'-CCGG-AGAGGTGTCGGCCATGGTTAT-CTCGAG-ATAACCATGGCCGACACCTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGTGTCGGCCATGGTTATCTCGAGATAACCATGGCCGACACCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGTGTCGGCCATGGTTATCTCGAGATAACCATGGCCGACACCTCT-3'

Other clones with same target sequence:

(none)