Construct: shRNA TRCN0000438367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199021.3-2237s21c1
DNA Barcode:
GGCATCCAGTGTACTGCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpp10 (269109)
Gene Description:
dipeptidylpeptidase 10
Transcript:
RefSeq NM_199021.3 (CURRENT)
Match location:
Position 2237 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCATCCAGTGTACTGCATAA
Hairpin Sequence:
5'-CCGG-GGCATCCAGTGTACTGCATAA-CTCGAG-TTATGCAGTACACTGGATGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCATCCAGTGTACTGCATAACTCGAGTTATGCAGTACACTGGATGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCATCCAGTGTACTGCATAACTCGAGTTATGCAGTACACTGGATGCC-3'

Other clones with same target sequence:

(none)