Construct: shRNA TRCN0000438393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172948.3-854s21c1
DNA Barcode:
ATGGTGAAGCGCATGGATATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mgat5b (268510)
Gene Description:
mannoside acetylglucosaminyltransferase 5, isoenzyme B
Transcript:
RefSeq NM_172948.3 (CURRENT)
Match location:
Position 854 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGTGAAGCGCATGGATATG
Hairpin Sequence:
5'-CCGG-ATGGTGAAGCGCATGGATATG-CTCGAG-CATATCCATGCGCTTCACCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGTGAAGCGCATGGATATGCTCGAGCATATCCATGCGCTTCACCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGTGAAGCGCATGGATATGCTCGAGCATATCCATGCGCTTCACCAT-3'

Other clones with same target sequence:

(none)