Construct: shRNA TRCN0000438439

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012276.3-297s21c1
DNA Barcode:
GGTCAAACTCTCCATCCCATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LILRA4 (23547)
Gene Description:
leukocyte immunoglobulin like receptor A4
Transcript:
RefSeq NM_012276.3 (NON-CURRENT)
Match location:
Position 297 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438439)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCAAACTCTCCATCCCATC
Hairpin Sequence:
5'-CCGG-GGTCAAACTCTCCATCCCATC-CTCGAG-GATGGGATGGAGAGTTTGACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCAAACTCTCCATCCCATCCTCGAGGATGGGATGGAGAGTTTGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCAAACTCTCCATCCCATCCTCGAGGATGGGATGGAGAGTTTGACC-3'

Other clones with same target sequence:

(none)