Construct: shRNA TRCN0000438441

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183027.2-617s21c1
DNA Barcode:
CCATGCTGTGAACCTACAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap1s3 (252903)
Gene Description:
adaptor-related protein complex AP-1, sigma 3
Transcript:
RefSeq NM_183027.2 (CURRENT)
Match location:
Position 617 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438441)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCTGTGAACCTACAAAC
Hairpin Sequence:
5'-CCGG-CCATGCTGTGAACCTACAAAC-CTCGAG-GTTTGTAGGTTCACAGCATGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCTGTGAACCTACAAACCTCGAGGTTTGTAGGTTCACAGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCTGTGAACCTACAAACCTCGAGGTTTGTAGGTTCACAGCATGG-3'

Other clones with same target sequence:

(none)