Construct: shRNA TRCN0000438447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014961.3-1323s21c1
DNA Barcode:
AGGACGGGAACAGCAGTAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RUFY3 (22902)
Gene Description:
RUN and FYVE domain containing 3
Transcript:
RefSeq NM_014961.3 (NON-CURRENT)
Match location:
Position 1323 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACGGGAACAGCAGTAAAG
Hairpin Sequence:
5'-CCGG-AGGACGGGAACAGCAGTAAAG-CTCGAG-CTTTACTGCTGTTCCCGTCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACGGGAACAGCAGTAAAGCTCGAGCTTTACTGCTGTTCCCGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACGGGAACAGCAGTAAAGCTCGAGCTTTACTGCTGTTCCCGTCCT-3'

Other clones with same target sequence:

(none)