Construct: shRNA TRCN0000438484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015004.3-418s21c1
DNA Barcode:
AGCACTGCTGGGTTCTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXOSC7 (23016)
Gene Description:
exosome component 7
Transcript:
RefSeq NM_015004.3 (NON-CURRENT)
Match location:
Position 418 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCACTGCTGGGTTCTCTATG
Hairpin Sequence:
5'-CCGG-AGCACTGCTGGGTTCTCTATG-CTCGAG-CATAGAGAACCCAGCAGTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCACTGCTGGGTTCTCTATGCTCGAGCATAGAGAACCCAGCAGTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCACTGCTGGGTTCTCTATGCTCGAGCATAGAGAACCCAGCAGTGCT-3'

Other clones with same target sequence:

(none)