Construct: shRNA TRCN0000438502

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144841.3-573s21c1
DNA Barcode:
AGGGTGCAGGTATGGTTTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Otx2 (18424)
Gene Description:
orthodenticle homeobox 2
Transcript:
RefSeq NM_144841.3 (NON-CURRENT)
Match location:
Position 573 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438502)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGTGCAGGTATGGTTTAAG
Hairpin Sequence:
5'-CCGG-AGGGTGCAGGTATGGTTTAAG-CTCGAG-CTTAAACCATACCTGCACCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGTGCAGGTATGGTTTAAGCTCGAGCTTAAACCATACCTGCACCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGTGCAGGTATGGTTTAAGCTCGAGCTTAAACCATACCTGCACCCT-3'

Other clones with same target sequence:

(none)