Construct: shRNA TRCN0000438526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000746.3-359s21c1
DNA Barcode:
GGGTGAAGACTGTTCGTTTCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHRNA7 (1139)
Gene Description:
cholinergic receptor nicotinic alpha 7 subunit
Transcript:
RefSeq NM_000746.3 (NON-CURRENT)
Match location:
Position 359 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTGAAGACTGTTCGTTTCC
Hairpin Sequence:
5'-CCGG-GGGTGAAGACTGTTCGTTTCC-CTCGAG-GGAAACGAACAGTCTTCACCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTGAAGACTGTTCGTTTCCCTCGAGGGAAACGAACAGTCTTCACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTGAAGACTGTTCGTTTCCCTCGAGGGAAACGAACAGTCTTCACCC-3'

Other clones with same target sequence:

(none)