Construct: shRNA TRCN0000438565

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002570.3-3007s21c1
DNA Barcode:
GTCGAAGGTGTGACGAGAACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCSK6 (5046)
Gene Description:
proprotein convertase subtilisin/kexin type 6
Transcript:
RefSeq NM_002570.3 (NON-CURRENT)
Match location:
Position 3007 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438565)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGAAGGTGTGACGAGAACT
Hairpin Sequence:
5'-CCGG-GTCGAAGGTGTGACGAGAACT-CTCGAG-AGTTCTCGTCACACCTTCGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGAAGGTGTGACGAGAACTCTCGAGAGTTCTCGTCACACCTTCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGAAGGTGTGACGAGAACTCTCGAGAGTTCTCGTCACACCTTCGAC-3'

Other clones with same target sequence:

(none)