Construct: shRNA TRCN0000438803

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006585.2-1571s21c1
DNA Barcode:
ACATGCTGGAAGCTGGTATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCT8 (10694)
Gene Description:
chaperonin containing TCP1 subunit 8
Transcript:
RefSeq NM_006585.2 (NON-CURRENT)
Match location:
Position 1571 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438803)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATGCTGGAAGCTGGTATTC
Hairpin Sequence:
5'-CCGG-ACATGCTGGAAGCTGGTATTC-CTCGAG-GAATACCAGCTTCCAGCATGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATGCTGGAAGCTGGTATTCCTCGAGGAATACCAGCTTCCAGCATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATGCTGGAAGCTGGTATTCCTCGAGGAATACCAGCTTCCAGCATGT-3'

Other clones with same target sequence:

(none)