Construct: shRNA TRCN0000438879

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172818.2-942s21c1
DNA Barcode:
TACGGGAAGACAGCATCATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttll8 (239591)
Gene Description:
tubulin tyrosine ligase-like family, member 8
Transcript:
RefSeq NM_172818.2 (NON-CURRENT)
Match location:
Position 942 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438879)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGGGAAGACAGCATCATTC
Hairpin Sequence:
5'-CCGG-TACGGGAAGACAGCATCATTC-CTCGAG-GAATGATGCTGTCTTCCCGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGGGAAGACAGCATCATTCCTCGAGGAATGATGCTGTCTTCCCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGGGAAGACAGCATCATTCCTCGAGGAATGATGCTGTCTTCCCGTA-3'

Other clones with same target sequence:

(none)