Construct: shRNA TRCN0000438889

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006953.3-757s21c1
DNA Barcode:
ACGACTCACGACTCCCAAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UPK3A (7380)
Gene Description:
uroplakin 3A
Transcript:
RefSeq NM_006953.3 (NON-CURRENT)
Match location:
Position 757 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000438889)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGACTCACGACTCCCAAATC
Hairpin Sequence:
5'-CCGG-ACGACTCACGACTCCCAAATC-CTCGAG-GATTTGGGAGTCGTGAGTCGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGACTCACGACTCCCAAATCCTCGAGGATTTGGGAGTCGTGAGTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGACTCACGACTCCCAAATCCTCGAGGATTTGGGAGTCGTGAGTCGT-3'

Other clones with same target sequence:

(none)