Construct: shRNA TRCN0000439038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015137.3-2205s21c1
DNA Barcode:
GCTAGGTGGAAGTGGATATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFR3A (23167)
Gene Description:
EFR3 homolog A
Transcript:
RefSeq NM_015137.3 (NON-CURRENT)
Match location:
Position 2205 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000439038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGGTGGAAGTGGATATAG
Hairpin Sequence:
5'-CCGG-GCTAGGTGGAAGTGGATATAG-CTCGAG-CTATATCCACTTCCACCTAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGGTGGAAGTGGATATAGCTCGAGCTATATCCACTTCCACCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGGTGGAAGTGGATATAGCTCGAGCTATATCCACTTCCACCTAGC-3'

Other clones with same target sequence:

(none)