Construct: shRNA TRCN0000439084

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011700.2-148s21c1
DNA Barcode:
GAAAGGGCTCATGGGAATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vill (22351)
Gene Description:
villin-like
Transcript:
RefSeq NM_011700.2 (CURRENT)
Match location:
Position 148 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000439084)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGGGCTCATGGGAATTTC
Hairpin Sequence:
5'-CCGG-GAAAGGGCTCATGGGAATTTC-CTCGAG-GAAATTCCCATGAGCCCTTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGGGCTCATGGGAATTTCCTCGAGGAAATTCCCATGAGCCCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGGGCTCATGGGAATTTCCTCGAGGAAATTCCCATGAGCCCTTTC-3'

Other clones with same target sequence:

(none)