Construct: shRNA TRCN0000439162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021139.2-521s21c1
DNA Barcode:
GCTGCTGGCCGAGTTACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT2B4 (7363)
Gene Description:
UDP glucuronosyltransferase family 2 member B4
Transcript:
RefSeq NM_021139.2 (NON-CURRENT)
Match location:
Position 521 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000439162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTGGCCGAGTTACTTAA
Hairpin Sequence:
5'-CCGG-GCTGCTGGCCGAGTTACTTAA-CTCGAG-TTAAGTAACTCGGCCAGCAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTGGCCGAGTTACTTAACTCGAGTTAAGTAACTCGGCCAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTGGCCGAGTTACTTAACTCGAGTTAAGTAACTCGGCCAGCAGC-3'

Other clones with same target sequence:

(none)