Construct: shRNA TRCN0000439204

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177718.3-1969s21c1
DNA Barcode:
GCATGCTGGCTGCGTTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mb21d2 (239796)
Gene Description:
Mab-21 domain containing 2
Transcript:
RefSeq NM_177718.3 (CURRENT)
Match location:
Position 1969 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000439204)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGCTGGCTGCGTTTATTT
Hairpin Sequence:
5'-CCGG-GCATGCTGGCTGCGTTTATTT-CTCGAG-AAATAAACGCAGCCAGCATGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGCTGGCTGCGTTTATTTCTCGAGAAATAAACGCAGCCAGCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGCTGGCTGCGTTTATTTCTCGAGAAATAAACGCAGCCAGCATGC-3'

Other clones with same target sequence:

(none)