Construct: shRNA TRCN0000439520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013231.4-1376s21c1
DNA Barcode:
CACGAGCTTGGAGCGTCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLRT2 (23768)
Gene Description:
fibronectin leucine rich transmembrane protein 2
Transcript:
RefSeq NM_013231.4 (NON-CURRENT)
Match location:
Position 1376 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000439520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGAGCTTGGAGCGTCTTAT
Hairpin Sequence:
5'-CCGG-CACGAGCTTGGAGCGTCTTAT-CTCGAG-ATAAGACGCTCCAAGCTCGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGAGCTTGGAGCGTCTTATCTCGAGATAAGACGCTCCAAGCTCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGAGCTTGGAGCGTCTTATCTCGAGATAAGACGCTCCAAGCTCGTG-3'

Other clones with same target sequence:

(none)