Construct: shRNA TRCN0000439641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006932.3-2507s21c1
DNA Barcode:
GCGCAAGGCCATGATTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMTN (6525)
Gene Description:
smoothelin
Transcript:
RefSeq NM_006932.3 (NON-CURRENT)
Match location:
Position 2507 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000439641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCAAGGCCATGATTGAGAA
Hairpin Sequence:
5'-CCGG-GCGCAAGGCCATGATTGAGAA-CTCGAG-TTCTCAATCATGGCCTTGCGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCAAGGCCATGATTGAGAACTCGAGTTCTCAATCATGGCCTTGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCAAGGCCATGATTGAGAACTCGAGTTCTCAATCATGGCCTTGCGC-3'

Other clones with same target sequence:

(none)