Construct: shRNA TRCN0000439777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017749.2-3975s21c1
DNA Barcode:
ACGGAACAGGTAGAGACAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMBRA1 (55626)
Gene Description:
autophagy and beclin 1 regulator 1
Transcript:
RefSeq NM_017749.2 (NON-CURRENT)
Match location:
Position 3975 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000439777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGAACAGGTAGAGACAAAC
Hairpin Sequence:
5'-CCGG-ACGGAACAGGTAGAGACAAAC-CTCGAG-GTTTGTCTCTACCTGTTCCGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGAACAGGTAGAGACAAACCTCGAGGTTTGTCTCTACCTGTTCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGAACAGGTAGAGACAAACCTCGAGGTTTGTCTCTACCTGTTCCGT-3'

Other clones with same target sequence:

(none)